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KMID : 0545120100200071114
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Journal of Microbiology and Biotechnology
2010 Volume.20 No. 7 p.1114 ~ p.1120
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Isolation, Characterization, and Application of a Chitosan-Degrading Fungus from Soil
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Xinlin Wei
Wei Chen
Ming Xiao
Jianbo Xiao
Yuanfeng Wang
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Abstract
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A chitosan-degrading fungus BSF114 was isolated from soil. The culture preparation showed strong chitosanolytic enzyme activity at optimum pH 4.0 and optimum temperature of 60 ºC after 36-40 h fermentation. The rapid decreased viscosity of chitosan solutions at early stage of reaction suggested the endo-type of cleavage in polymeric chitosan chains. To identify this fungus, both molecular biological and morphological methods were used. Fungal internal transcribed spacer (ITS) region 1 was amplified, sequenced, and then compared with relative sequences at GenBank database by BLAST. Phylogenetic relationships were then analyzed and the result showed this fungus belongs to Aspergillus fumigatus. Furthermore, the morphological observations were used to confirm above conclusions. The chitooligosaccharides obtained through hydrolyzing the colloidal chitosan showed A. fumigatus BSF114 is suitable to degrade the chitosan and produce chitooligosaccharides in large scale. High concentration of COS (1000 and 500 μg/mL) significantly proliferated the mice marrow cells.
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KEYWORD
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Chitooligosaccharides, Identification, Morphology, Aspergillus fumigatus
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